We co-stained sections of postmortem motor cortex with published, validated antibodies specific to human GDE2 (Nakamura et al, 2021) and TDP-43 focusing on tissue from patients with ALS because of the prevalence of TDP-43 pathologies in ALS and tissue availability (see Appendix Table S1 for demographic information). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.