Additionally, mice treated with anti–TIM-3 Ab in the absence of allo-HCT were not protected from FLT3-ITD MLL-PTD–driven AML (Supplemental Figure 2A), supporting the hypothesis that anti–TIM-3 Ab have no direct cytotoxic effect against AML cells, but require allo-HCT. This evidence concerns the gene KMT2A and acute myeloid leukemia.