Given that by day 20AE, HSD-fed flies demonstrated several hallmark features of T2D, fibrosis-like accumulation of Prc in the cardiac ECM, and cardiac dysfunction, as evidenced earlier (Musselman et al., 2011; Na et al., 2013), for the rest of the study, all analyses were done on day 20AE. This evidence concerns the gene PPRC1 and type 2 diabetes mellitus.