Genes where known chemical inhibitors were available were chosen as targets in the first instance and a chemical inhibitor was identified for a number of these Signature one gene products that could be used to test their role in LF82rpsmGFP infection; ST034307 for Adcy1, clomipramine for Itch, trametinib for Map2k1, necrosulfonamide for Mlkl, and GSK2636771 for Pik3cb (Table 2). This evidence concerns the gene MAP2K1 and infection.