For the prostate cancer sample, the same trajectory inference methodology (Fig. 1u and Supplementary Fig. S3a) also identified three branches (Supplementary Fig. S3b–d), although less defined, and a glmnet analysis revealed the genes associated with these phenotypic paths and distinguished them by their expression of ODC1, SPON2, ADGRF1, TSPAN8, CLDN3, and KRT8 among others (Supplementary Fig. S3e and f). Here, TSPAN8 is linked to Familial prostate cancer.