Investigations by Qi et al., through single-cell RNA sequencing, uncovered that low expression of GPR65 in osteosarcoma is prognostically unfavorable, and that its expression is positively connected with the enhanced activity and infiltration of immune cells, especially CD4+ and CD8+ T cells, that obstruct osteosarcoma progression (Qi et al., 2024). This evidence concerns the gene CD4 and osteosarcoma.