In addition, research conducted by Liu et al. elucidated modifications in the osteosarcoma immune microenvironment, specifically the perturbation of peripheral blood T lymphocyte subsets during osteosarcoma development, characterized by an increase in γδ T cells and Tregs, a decrease in CD8+ T cells, concomitant with peripheral immune suppression (Liu Q. et al., 2022). This evidence concerns the gene CD8A and osteosarcoma.