Subsequent investigations revealed that inhibition of c-Myc in osteosarcoma augmented the tumor immune microenvironment by elevating T cell chemokines and stimulating the CD40/CD40L pathway, facilitating the infiltration and activation of CTLs, highlighting the positive contribution of T cells in osteosarcoma treatment (Jiang et al., 2022). This evidence concerns the gene CD40LG and osteosarcoma.