Competitive radioligand saturation-binding assays with [89Zr]Zr-DFO-avelumab, demonstrated that NCIN87 cancer cells express 44,000 PD-L1 per cell (Figure 2B), which is higher than previous studies in the gastric cancer cell line MKN45 (<2,200 PD-L1 per cell) (28). This evidence concerns the gene CD274 and cancer.