Of particular interest, our analysis of PICALM levels does not recapitulate previous studies, which show PICALM isoform 4 as both increased (Ando et al., 2016) or decreased (Ando et al., 2013; Alsaqati et al., 2023) in AD depending on if the soluble or insoluble protein fraction was extracted. This evidence concerns the gene PICALM and Alzheimer disease.