In multivariable logistic regression analysis, the age of participants during viral load test ≥ 35 Years, being single in marital status, AZT + 3TC + NVP regimen as baseline ART, Base-line CD4 < 350 mm3, having a history of TB co-infection, and having opportunistic infection other than TB in last 6 months were significantly associated with Virologic failure and having a history of ASM were inversely associated with Virologic failure (Table 1). This evidence concerns the gene CD4 and coinfection.