Individuals with unhealthy sleep and high level of ALT (HR = 3.65, 95% CI: 2.43, 5.48), AST (HR = 4.03, 95% CI: 2.69, 6.03), TBIL (HR = 1.97, 95% CI: 1.40, 2.77), GGT (HR = 4.69, 95% CI: 2.98, 7.37), ALP (HR = 2.51, 95% CI: 1.75, 3.59), or TP (HR = 2.09, 95% CI: 1.51, 2.89) or low level of ALB (HR = 2.22, 95% CI:1.55, 3.17) were at the highest risk for liver cancer incidence compared with those with healthy sleep and low level of ALT, AST, TBIL, GGT, ALP, or TP or high level of ALB. This evidence concerns the gene GPT and liver cancer.