Based on 235 hepatocellular carcinoma (HCC) patients and 259 healthy controls with negative alpha-fetoprotein, Li and colleagues found that GGT/ALP combined with GGT/ALT and ALT/AST were effective diagnostic markers of alpha-fetoprotein-negative HCC, especially in patients with normal liver function or early stage [17]. This evidence concerns the gene AFP and hepatocellular carcinoma.