We annotated and distinguished different cell populations using specific markers (ALDH1A1 and ALB for epithelial cells and tumor cells, CD79A and MS4A1 for B cells, CD3D and CD3E for T cells, FGFBP2 for NK cells, CD33 for granulocytes, CD68, CD163, and CD14 for macrophages, ITGAX for dendritic cells, ACTA2 and COL1A2 for fibroblasts, PECAM1 and vWF for endothelial cells) (Fig. 2A-B) [19]. The gene discussed is ACTA2; the disease is neoplasm.