We stained 873 HCC specimens with CD4, CD68, CD8, CD66b, and Foxp3 antibodies, revealing distinct immune cell types in the TME, where CD8 signifies cytotoxic T cells, CD66b is a surface marker of TAN, and Foxp3 represents Treg (Fig. 3A). Here, FOXP3 is linked to hepatocellular carcinoma.