VHL and breast neoplasm: To evaluate the impact of VHL on UBE3B-mediated breast tumor growth and metastasis in vivo, control, Flag-VHL, and Flag-VHL + Myc-UBE3B MDA-MB-231 cells were implanted into the mammary fat pad of female nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice, respectively.