Interestingly, in chronically epileptic female Kcc2/Crh KO mice, seizure frequency and seizure burden remained reduced in the post CNO off period (Fig. 5E,G), and treatment with Gi DREADDs resulted in no SUDEP incidence in the female Kcc2/Crh KO mice (Fig. 6F). This evidence concerns the gene SLC12A5 and Sudden unexpected death in epilepsy.