More importantly, 22 and 33 not onlydemonstrated remarkable in vitro inhibitory potencyat the single-digit nanomolar level but also exhibited excellent immune-modulatingactivity by enhancing the expression of total T-cells, cytotoxic CD8+ T-cells, and helper CD4+ T-cells in the spleen.Additionally, 33 also remarkably exhibited anticanceractivity, achieving a TGI value of nearly 90% at 50 mg/kg BID orallyin the MC38 syngeneic murine colorectal model. The gene discussed is CD8A; the disease is medical procedure.