The only subgroup of patients in whom metabolic indices were altered when compared with controls was that comprised of women showing hyperandrogenemia by LC–MS/MS, regardless of immunoassays; these patients presented with reduced  insulin sensitivity index and HDL-cholesterol, and increased diastolic BP values and triglycerides concentrations, and were more likely to have an abnormal glucose metabolism, namely prediabetes or diabetes mellitus (Table 4). This evidence concerns the gene INS and prediabetes syndrome.