ACTA1 and myocardial infarction: Masson-Trichrome staining and RT-qPCR revealed that compared to the sham-operated mice, mice subjected to MI surgery for a duration of 4 weeks exhibited a noticeable MI scar, disordered myocardial fibers and cardiac fibrosis, along with increased expression levels of α-SMA and collagen I, the well-established fibroblast marker for the differentiation of fibroblast to myofibroblast and a marker for the accumulation of extracellular matrix proteins, which were not affected by the intramyocardial delivery of lentivirus-encoded scramble sequence.