Additionally, both macrophages, Alpl+ and Mgp+ dural cells were presented in the regenerative tissue of the C15G85-PDA graft at 2 weeks, and further crosstalk between dural cells and macrophages was confirmed by CellPhoneDB analysis, while macrophage depletion by clodronate liposome resulted in the significant reduction of macrophages, Alpl+ and Mgp+ dural cells, which resulted in little bone healing. Here, ALPL is linked to Patent ductus arteriosus.