MUC1 and cancer: The replication of TILT-322 in human MUC1-expressing A549 cells supernatants is shown in Figure S1A. Furthermore, TILT-322 exhibited the ability to effectively target and kill a range of human cancer cells expressing MUC1, including A549, T47D, PDX-OvCa, MDAMB-231, and PC3MM2, with comparable efficacy to the unarmed backbone virus (Figures S1B–S1F).