To clarify the relationship between CTSE gene and BLCA, the CTSE overexpression was constructed in two human bladder transitional cell carcinoma cells lines T24 and J82 by transfecting with Ad-CTSE adenovirus (Fig. 12A,B), and the expression level of CTSE in the transfected group increased significantly after the successful construction (Fig. 12C,D). Here, CTSE is linked to bladder transitional cell carcinoma.