In the present study, our results exhibited a disruption of the tight junctional integrity of corneal endothelium in SD-treated mice, such as remarkedly decreased ZO-1 expression, which is similar to the pathological changes in corneal epithelium described in the dry eye induced by SD lasting for 10 days in mouse models [6]. Here, TJP1 is linked to Keratoconjunctivitis sicca.