To validate the methylation-dependent expression of HSP47 in GC, we further analyzed the effect of the demethylating agent 5’-aza-2’-deoxycytidine (5-Aza-dC) on the low HSP47-expressing cell lines SNU-216 and SNU-719, as established in Fig. 1A. HSP47 expression increased in SNU-216 and SNU-719 GC cells treated with 5-Aza-dC, as evidenced by qRT-PCR analysis (1.7- and 3.0-fold increases, respectively) (Fig. 6C). This evidence concerns the gene SERPINH1 and gastric cancer.