Interestingly, there was no significant correlation with tumor purity, B cell, CD8 + T cell, neutrophil and the expression of NOTCH3, despite the temporary relationship between CD4 + T cell (cor = − 0.389, P = 1.14e−14), macrophage (cor = − 0.326, P = 1.31e−10), dendritic cell (cor = − 0.18, P = 4.84e−04) infiltration and NOTCH3 levels in STAD. This evidence concerns the gene CD4 and neoplasm.