Importantly, after adjusting for grade, stage, serum CEA, and serum CA199, multivariate Cox regression analysis demonstrated that increased RGS16 expression in CRC served as a statistically significant predictor of DFS (2.278, 95% CI: 1.693–3.067) and DSS (2.615, 95% CI: 1.937–3.530). Here, RGS16 is linked to colorectal carcinoma.