GSDMD and ischemia: To demonstrate whether a specific cytokine plays a role in angiogenesis after hindlimb ischemia, we measured mice serum cytokine levels using Luminex technology in four groups: WT sham (n = 3), WT HLI (n = 4), KO sham (n = 3), and KO HLI (n = 4) to compare cytokine releasing levels between WT mice and GSDMD-/- mice after HLI (Fig. 6A).