Analysis by QUANTISEQ and XCELL algorithms further confirmed that NPC tumours with higher PJA1 expression were infiltrated with fewer DCs and CD8+ T cells than those with lower PJA1 expression (Supplementary Fig. 5b, c), while the infiltration of M1 macrophages and neutrophils did not differ significantly between these tumours (Supplementary Fig. 5d). The gene discussed is PJA1; the disease is nasopharyngeal carcinoma.