To validate the effect of PJA1 on the infiltration of immune cells, we performed IHC staining and multiplex IF to detect PJA1 expression and CD3+ cells, CD8+ T cells and CD11c+ DCs infiltration in 50 NPC tissue samples with low (n = 23) or high (n = 27) PJA1 expression (Fig. 6b). The gene discussed is PJA1; the disease is nasopharyngeal carcinoma.