G3BP1 and esophageal squamous cell carcinoma: To identify the essential role of Wnt/β-Catenin pathway in USP21/G3BP1 axis-regulated ESCC progression, IWR-1, an inhibitor for Wnt/β-Catenin signaling, was applied to treat KYSE-150 cells with USP21 overexpression, while laduviglusib, an activator for Wnt/β-Catenin signaling, was added into culture medium of KYSE-150 cells with USP21 depletion.