To further identify the function of G3BP1 in USP21-mediated progression of ESCC, we transfected G3BP1-expressing plasmids to KYSE-150 and Eca-109 cells with siRNA-regulated USP21 knockdown, as shown in Fig. 4E and Fig. S8E. This evidence concerns the gene G3BP1 and esophageal squamous cell carcinoma.