Similarly, treatment with TMZ (Fig. 1g, h and Supplementary Fig. 1f), etoposide (Fig. 1i, j and Supplementary Fig. 1g), and doxorubicin (Fig. 1k, l and Supplementary Fig. 1h) increased activation of both Akt and ERK signaling in multiple Ewing sarcoma cells in vitro. Here, AKT1 is linked to Ewing sarcoma.