To explore the role of KCNK1 in breast cancer, KCNK1 was overexpressed or knocked down in MDA-MB-231, MDA-MB-468, MCF-7, and T47D cells by transfecting the KCNK1 overexpression construct or 2 KCNK1-specific shRNAs, respectively (Figs 2A, 2B, S2A, and S2B). This evidence concerns the gene KCNK1 and breast cancer.