To assess the expression and correlation among KCNK1, LDHA, Pan Kla, ZWINT, ECT2, ANLN, and EZR, immunohistochemistry was performed using 18 pairs of paraffin-embedded breast cancer and adjacent normal breast tissue samples (S2 Table), the data showed that KCNK1 was highly differentially expressed in breast cancer samples, and the expression of LDHA, Pan Kla, ZWINT, ECT2, ANLN, and EZR were also higher than that in those paired adjacent normal breast tissues (Fig 8A and 8B). The gene discussed is ANLN; the disease is breast cancer.