INS and obesity due to melanocortin 4 receptor deficiency: Animal studies have shown that fasting in diabetic mice can downregulate the expression of inflammatory factors, thereby alleviating inflammation.28 A 5:2 MR plan may reshape the gut microbiota, promote white adipose tissue browning, and consequently reduce insulin resistance and the occurrence of obesity.29,30 The MR used in this study contained omega-3 fatty acids and medium-chain fatty acids.