In the present study, we show that POLG astrocytes in vitro exhibit uniform polarization into LPS-induced neuroinflammation A1 subtype nor ischemia-induced A2-reactive states, and display a mixed phenotype characterized by the up-regulation of GFAP and α-SMA/NESTIN, two cytoskeletal proteins expressed during astrocyte development. Here, GFAP is linked to ischemia.