Interestingly, macrophages were labeled with CD68, and immunohistochemical results showed more macrophage infiltration in RA synovium (Fig. 3 a-b), while immunofluorescence results showed more co-localization of macrophages NLRP3 and CD68 in RA synovium (Fig. 3 c-d). This evidence concerns the gene NLRP3 and rheumatoid arthritis.