Furthermore, scRNA-seq identified 4 different macrophage subpopulations: resident M1, M2, M3, and infiltrating macrophages in kidneys of FA-treated mice, with higher proportion of resident M2 macrophages and lower proportion of resident M3 macrophages in Aoah-/- mice after FA treatment (Figure 5B and Figure S3C). Here, AOAH is linked to Friedreich ataxia.