A DEG analysis between these distinct MC38 cancer cell populations in vivo showed that cells more similar to SIS ECM MatriSpheres upregulated histone genes (Hist2h2ac, Hist1h1a, Hist1h1b, Hist1h1e, Hist1h4d, Hist1h2ae) and proto-oncogene, Mybl2, and downregulated ECM protein and remodeling genes (Col3a1, Col1a1, Mmp2). This evidence concerns the gene H1-5 and cancer.