Here, we used RNA-Seq to study gene changes in mice with lipopolysaccharides (LPS)-induced ARDS and LPS-treated CtBP2-knockdown (CtBP2KD), p300KD, and p65KD cells, emphasizing the CtBP2-p300-NF-κB specificity in proinflammatory gene regulation. The gene discussed is NFKB1; the disease is acute respiratory distress syndrome.