Additionally, qRT-PCR, Western blot, and immunofluorescence (IF) analyses were performed to assess the expression of KIAA1429 in six GC cell lines (AGS, MGC-803, HGC-27, KATO III, MKN-45, and NCI-N87) and the immortalized normal gastric epithelial cell line GES-1. Here, VIRMA is linked to gastric cancer.