Another study showed that SFN also inhibited glucokinase 2 (HK2) and pyruvate dehydrogenase (PDH), decreased glycolysis and mitochondrial oxidative phosphorylation, blocked the Akt1/HK2 axis, and reduced aerobic glycolysis with abnormal glucose transport in bladder cancer cells and BBN-induced bladder tumor mouse (Huang et al. 2022). This evidence concerns the gene HK2 and urinary bladder cancer.