LGR5 and adenoma: We bred mice carrying lox alleles at the tumor suppressors and oncogenes most frequently mutated along the adenoma-to-carcinoma sequence, namely Apc14, Kras15 and Trp53 (encoding p53)16, each combined with Cre specific for Lgr5+ ISCs (Lgr5CreERT2-EGFP)17 or for PCs (Lyz1CreERT2)18.