To elucidate the underlying mechanism of how ALKBH4 inhibits pyroptosis in gastric cancer cells, we evaluated the impact of ALKBH4 expression on key targets (GSDME, GSDMD, NLRP3, Caspase 1) of the pyroptosis pathway using qPCR and western blot experiments. The gene discussed is ALKBH4; the disease is gastric cancer.