The PPI network of 27 proteins commonly associated with Dlat, Pdhb, and Pdhα is shown in Fig. 3C. Additionally, the KEGG analysis demonstrated that genes regulated by Dlat, Pdhb and Pdhα were primarily enriched in pyruvate metabolism, propanoate metabolism, metabolic pathways, HIF-1 signaling pathway, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, glucagon signaling pathway, citrate cycle (TCA cycle), central carbon metabolism in cancer, carbon metabolism, biosynthesis of amino acids, and 2-oxocarboxylic acid metabolism (Fig. 3D). This evidence concerns the gene PDHB and cancer.