The analysis of the function of cuproptosis-related genes in the MI/RI, through the Venn diagram, revealed that cuproptosis-related genes (Dlat, Pdhb, and Pdhα) belonged to the 768 down-regulated genes in the myocardial tissue of the ischemia-reperfusion injury mice (Fig. 3A). Here, PDHA1 is linked to ischemia reperfusion injury.