Although there is increasing evidence that VPS4 and ESCRT have no dominant proviral role during HCMV infection, VPS4 and other ESCRT components such as CHMP1 and HRS localize in the vicinity of the cytoplasmic viral assembly compartment (cVAC) where HCMV viral assembly and maturation in the cytoplasm takes place [20–23]. This evidence concerns the gene VPS4A and cytomegalovirus infection.