NF1 and sarcoma: We also performed qPCR using Nf1 and p53 specific primers on normal sciatic nerves (no injury) from wild-type mice (n = 2), injury-induced sciatic nerves from NPcis mice that develop pNF (n = 2), injury-induced sciatic nerves from NPcis mice that did not develop (n = 2) pNF and spontaneous sarcoma from NPcis mice (n = 2).