Given the hyperphagia and obesity in Ob/Ob mice, we matched body weights of Con and Ob/Ob mice by maintaining Ob/Ob mice on RD and the Con mice on HFD (Figure 6B) as conducted previously.22–24 As noted in Figures 1B and 1C, Con TAN-fed mice (compared to Con ad-lib mice) displayed reduced fat mass (with corresponding increases in fat-free/lean mass) (Figure 6C), decreased sWAT weight (Figure 6D), increased sWAT OCR (Figure 6E), and increased ILC2 cells in sWAT (Figure 6F) without affecting other sWAT immune cells, including FOXP3-positive T regulatory (Treg) cells (Figures S6B–S6H). The gene discussed is FOXP3; the disease is obesity due to melanocortin 4 receptor deficiency.