In this study, we opted toinvestigate two injection routes of IFs, which was based on our previouslypublished in vivo work showcasing the therapeuticefficacy of IFs functionalized with pMHC in tumor mouse models.17 Since the aim of the therapy with IF-pMHC isto stimulate antigen-specific T cells in vivo, wefurther simulated a situation in which antigen-specific T cells arepresent by adoptively transferring OT-I CD8+ T cells. Here, CD8A is linked to neoplasm.