According to the results of the label‐free profiling and Western blotting results, we speculated that on the one hand, the nuclear entry of β‐catenin could be prevented by downregulating TNKS to reduce the activity of the Wnt pathway; on the other hand, the proliferation of lung adenocarcinoma cells could be inhibited by the Wnt/PLAN pathway. This evidence concerns the gene TNKS and lung adenocarcinoma.