In this study, by integrating a GC patient cohort, public datasets, and in vitro and in vivo models, we found that neutrophil-expressed Interferon Induced Protein with Tetratricopeptide Repeats 1 (IFIT1) promoted EMT in GC, particularly in PCC-GC, and promoted mesenchymal cell recruitment through multiple signals, which subsequently facilitated an exhausted phenotype of T cells and induced resistance to immunotherapy. Here, IFIT1 is linked to gastric cancer.