After testing mutation detection rate of 4 mutations of the UltraSEEK® Lung Panel on gDNA and non-fixed single cells (Fig. EV3A,C), we tested the detection of EGFR T790M and L858R mutations on single tumor cells from the H1975 cell line processed through the Parsortix® enrichment method and obtained between 57% (_f2) and 78.5% (_f1) detection rate for EGFR T790M mutation and 64.3% for EGFR L858R mutation (Fig. EV3B,C). This evidence concerns the gene EGFR and neoplasm.