We discovered 36 specific hypermethylated marks associated with an increased risk of BC, including cg47630224-MSH2, cg23652916-PALB2, cg89786999-FANCI, and cg47596828- EPCAM. These CpG sites had not previously been linked to BC risk, which underscores the benefit of using open platforms such as NGS for evaluating genome-wide DNA methylation status in familial BC. This evidence concerns the gene FANCI and breast cancer.