To further validate these observations, we performed IHC and multiplex immunofluorescence on sublevel tumor sections to characterize B cells (CD20), T cells (CD3), fibroblasts (SMA (smooth muscle actin), FAP (fibroblast activation protein)), macrophages (CD163, CD115, CD11b), and HLA-DR-positive cells. The gene discussed is FAP; the disease is neoplasm.