Using cutoff values based on the mean value plus 2 SD in healthy controls (0.3 for anti-Ro52 and 0.8 for anti-Ro60), the respective frequencies of anti-Ro52 and anti-Ro60 antibody positivity in different disease/autoantibody subsets were as follows: 36% (63/177) and 15% (27/177) of anti-Jo-1, 40% (8/20) and 20% (4/20) of non-Jo-1 anti-tRNA synthetase, 11% (2/18) and 10% (2/20) of anti-SRP, 10% (2/20), and 25% (5/20) of anti-topoisomerase I-positive patients—versus 30% (9/30) and 70% (21/30) of primary Sjogren’s syndrome patients. The gene discussed is TRIM21; the disease is Sjogren syndrome.